Supplementary movies for PhD thesis – Niv Ierushalmi
Movie 1 – Steady-state dynamics in a symmetric droplet. This movie shows spinning disc confocal images of a contracting actomyosin network labeled with GFP-Lifeact in a symmetric droplet (Fig. 3.1b,c, left). The droplet was squished in a 30µm-high chamber to improve the imaging. Images were taken at the equatorial plane.Movie 2. Steady-state dynamics in a polar droplet. This movie shows spinning disc confocal images of a contracting actomyosin network labeled with GFP-Lifeact in a polar droplet (Fig. 3.1b,c, right). The droplet was squished in a 30µm-high chamber to improve the imaging. Images were taken at the equatorial plane.Movie 3. Symmetry breaking in a droplet that transitions from a symmetric state into a polar state. The dynamics of the actin network labeled with GFP-Lifeact in a spherical droplet were followed by spinning disc confocal time lapse imaging. The movie shows a symmetry breaking process, in which the aggregate moves from the center of the droplet toward the side.Movie 4. Recentering dynamics following a magnetic perturbation. This movie shows spinning disc confocal and bright-field images of a magnetic perturbation experiment (Fig. 3.5b-d). The aggregate was pulled from the center using a magnetic needle, and subsequently recentered after the magnet is removed.Movie 5. Transition to a polar state following a magnetic perturbation. This movie shows spinning disc confocal and bright-field images of a magnetic perturbation experiment in which the droplet became polar following the perturbation (Fig. 3.6). The aggregate was pulled from the center using a magnetic needle, and subsequently continued moving in the same direction until it reached the boundary.Movie 6. Periodic contraction waves. This movie shows periodic actin contraction waves in a large extract droplet (Fig. 3.19). Actin filaments are labeled with lifeAct-GFP.Movie 7. Droplet with waves and continuous contraction. This movie shows a droplet with periodic contraction from the far side (top), and continuous contraction from the close side (bottom) (Fig. 3.22).Movie 8. Spiral wave. This movie shows a periodic spiral contraction wave (Fig. 3.24)